MilliporeSigma™ Mouse anti-CD1d, Clone: D5,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ MABC983

Catalog No. MABC983MI

Description

Specifically detects CD1d clone: D5 in Human samples, and it is validated for Immunoprecipitation, Western Blotting

Antigen-presenting glycoprotein CD1d (UniProt P15813; also known as CD1d, R3G1) is encoded by the CD1D gene (Gene ID 912) in human. CD1d is an MHC-like glycoprotein expressed on epithelial cells and can signal innate immune responses by presenting lipid antigen to natural killer (NK) and NK T (NKT) cells. Similar to MHC class I heavy chain (HC), CD1d HC is glycosylated and assembled with β2m in ER. Unlike MHC class I HC, misfoled CD1d and CD1d not properly assembled with β2m are not destroyed by ER quality control mechanisms. CD1d plays a role in both innate and adaptive immunity to various bacteria, viruses, fungi, and parasites. Activation of CD1d-restricted invariant NKT cells enhances host resistance to these microbes via direct killing of CD1d-expressing (infected) cells or by promoting interferon gamma release from conventional NK cells and by modulating adaptive immune cells via alteration of Th1/Th2 polarization. Chlamydia trachomatis is shown to prevent CD1d-mediated innate immune destruction of the infected urethral epithelial cells by targeting CD1d for proteolytic degradation. Specifically, the chlamydial proteasome-like activity factor (CPAF) interacts with the CD1d HC, and CPAF-associated CD1d HC is then ubiquitinated for eventual degradation.

Specifications

• Antigen: CD1d
• Classification: Monoclonal
• Formulation: Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
• Gene Symbols: CD1D
• Immunogen: Pooled CD1d-GST fusion proteins containing α1 domain alone, α1-α2 domains, or α1–α3 domains.
• Quantity: 200 μg
• Research Discipline: Apoptosis, Cancer
• Gene ID (Entrez): NP_001757
• Content And Storage: Stable for one year at 2°C to 8°C from date of receipt.
• Isotype: IgG2b κ

• Applications: Immunoprecipitation, Western Blot
• Clone: D5
• Gene Accession No.: P15813
• Host Species: Mouse
• Purification Method: Protein G Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human
• Form: Purified

For Research Use Only