CD25 Mouse anti-Human, APC-R700, Clone: 2A3, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 565107

Catalog No. BDB565107

Description

The 2A3 monoclonal antibody specifically binds to human CD25, the low-affinity alpha subunit of the Interleukin-2 Receptor (IL- 2Rα). CD25 associates with CD122 (IL-2Rβ chain) and CD132 (common γchain or γc) to form the high-affinity signal-transducing IL -2R complex. CD25 is expressed by subsets of thymocytes and peripheral blood lymphocytes including CD4+CD25+ regulatory T cells and memory T cells. CD25 antigen density increases on activated T cells including phytohemagglutinin (PHA)-, concanavalin A (Con A)-, and CD3-activated T lymphocytes. High levels of CD25 can be expressed by T lymphocytes from mixed lymphocyte cultures and by human T-lymphocyte leukemia virus (HTLV)-infected T-lymphocyte leukemia lines, for example, HUT-102. CD25 can also be expressed by activated B cells and macrophages. Recombinant IL-2 blocks the binding of the 2A3 antibody to PHA-activated T lymphocytes.

This antibody was conjugated to BD Horizon APC-R700, which has been developed exclusively by BD Biosciences as a better alternative to Alexa Fluor™ 700. APC-R700 excites and emits at similar wavelengths to Alexa Fluor™ 700 yet exhibits significantly improved brightness. This dye can be excited by the red laser and detected with the same filter set as Alexa Fluor™ (eg, 730/45-nm filter).

Specifications

• Antigen: CD25
• Classification: Monoclonal
• Conjugate: APC-R700
• Formulation: Aqueous buffered solution containing BSA, protein stabilizer, glycerol, and ≤0.09% sodium azide.
• Immunogen: Human Phytohemagglutinin-activated T Cells
• Quantity: 25 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Isotype: IgG1 κ

• Applications: Flow Cytometry
• Clone: 2A3
• Description: IL-2R; IL2RA; IL-2Rα; TCGFR; TAC antigen; p55
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Gene ID (Entrez): 100
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only.