Mouse anti-CD35, Clone: E11, Novus Biologicals™

Supplier:  Novus Biologicals NBP2294600.2MG

Catalog No. NBP22946002

Specifications

• Antigen: CD35
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Dilution: Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, ELISA 1-5ug/ml for coating, Immunohistochemistry 0.5-1ug/ml, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml, SDS-Page
• Gene Accession No.: P17927
• Gene Symbols: CR1
• Immunogen: Intact human monocytes were used as immunogen to generate the CD35 antibody (Hogg et al, 1984).
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Gene ID (Entrez): 1378
• Target Species: Human, Primate
• Form: Purified

• Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Clone: E11
• Conjugate: Unconjugated
• Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
• Gene Alias: complement component (3b/4b) receptor 1 (Knops blood group), KN
• Host Species: Mouse
• Molecular Weight of Antigen: 215 kDa
• Quantity: 0.2 mg
• Primary or Secondary: Primary
• Test Specificity: Recognizes a protein of 210-220kDa, which is identified as the complement receptor 1 (CR1)/CD35. This MAb is specific for a site in CR1 that is distal from the C3b-binding site, so that it is unable to block CR1 activity. This MAb is highly specific to CR1 and shows no cross-reaction with CR2. The primary function of CR1 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein. Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. They are CD21+/CD35+/CD1a-. This MAb labels follicular dendritic cells and follicular dendritic cell sarcoma.
• Content And Storage: Store at 4C.
• Isotype: IgG1 κ

For Research Use Only