MilliporeSigma™ Mouse anti-CD4, Clone: RPA-T4, PE,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ MABF1720

Catalog No. MABF1720

Description

Specifically detects CD4 clone: RPA-T4 in Human samples, and it is validated for Flow Cytometry

The RPA-T4 antibody reacts with human CD4, a 59kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4, and co-receptors CCR5 and CXCR4, may also be utilized by HIV-1 to enter T cells. Human CD4 is typically expressed on thymocytes, some mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.The RPA-T4 antibody is widely used as a phenotypic marker for human CD4 expression, and is cross-reactive with Chimpanzee CD4. This antibody recognizes a different epitope, and thus does not block binding of, the alternative Anti-Human CD4 antibody clone OKT4 (Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. 76:4061-4065).

Specifications

• Antigen: CD4
• Classification: Monoclonal
• Conjugate: PE
• Gene Accession No.: P01730
• Host Species: Mouse
• Purification Method: Purified by size-exclusion chromatography
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Stable for one year at 2°C to 8°C from date of receipt. Note: It is recommended to store the product undiluted at 2°C to 8°C and protected from prolonged exposure to light. Do not freeze.

• Applications: Flow Cytometry
• Clone: RPA-T4
• Formulation: Purified Mouse monoclonal containing 10mM NaH₂PO₄, 150mM NaCl, 0.09% NaN₃, 0.1% gelatin, pH 7.2.
• Gene Symbols: CD4
• Immunogen: Immunogen information for this antibody is proprietary information.
• Quantity: 500 Tests
• Research Discipline: Inflammation and Immunology
• Target Species: Human
• Form: Purified

For Research Use Only