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Mouse anti-CD43/Sialophorin, Clone: Bra7G, Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer:  Novus BiologicalsSupplier Diversity Partner NBP2446500.02MG

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Catalog No. NBP24465000


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Description

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

CD43/Sialophorin Monoclonal antibody specifically detects CD43/Sialophorin in Human samples. It is validated for Flow Cytometry, Immunohistochemistry-Paraffin, Immunofluorescence.
Specifications

Specifications

CD43/Sialophorin
Bra7G
Unconjugated
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
CD43 antigen, CD43), Galactoglycoprotein, GALGP, Leukocyte sialoglycoprotein, Sialophorin, sialophorin (gpL115, leukosialin, CD43)
Mouse
IgM Kappa
0.02 mg
Immunology
Primary
6693
Human
Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
0.2mg/mL
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 1 - 2 ug/ml
Purified
SPN
Immature pluripotent human leukemia K562 cells
PEG purified
RUO
Store at 4C.
Monoclonal
It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43 (Workshop V). Epitope of MAb Bra7G is clearly different from that of MAb DF-T1, called b as opposed to a for DF-T1. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.
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