CD59 Antibody (193-27), Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP24469800	Novus Biologicals NBP2446980.02MG	0.02 mg
NBP24469801	Novus Biologicals NBP2446980.1MG	0.1 mg
NBP24469802	Novus Biologicals NBP2446980.2MG	0.2 mg

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunofluorescence

CD59 Monoclonal specifically detects CD59 in Human, Baboon (Negative), Equine (Negative) samples. It is validated for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Functional, Immunofluorescence.

Specifications

• Antigen: CD59
• Concentration: 0.2 mg/mL
• Applications: Flow Cytometry, Immunofluorescence
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Cell Biology, Cellular Markers, Immunology, Signal Transduction, Stem Cell Markers
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene ID (Entrez): 966
• Immunogen: Stimulated human leukocytes
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.
• Molecular Weight of Antigen: 20 kDa

• Clone: 193-27
• Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunofluorescence 0.5 - 1.0 ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Baboon (Negative), Equine (Negative)
• Gene Alias: 16.3A5, 1F5, 1F5 antigen, 20 kDa homologous restriction factor, CD59 antigen, CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, CD59 antigen, complement regulatory protein, CD59 glycoprotein, CD59 molecule, complement regulatory protein, EJ16, EJ30, EJ30, EL32 and G344), EL32, FLJ38134, FLJ92039, G344, HRF20, HRF-20, human leukocyte antigen MIC11, Ly-6-like protein, lymphocytic antigen CD59/MEM43, MACIF, MAC-inhibitory protein, MAC-IP, MEM43, MEM43 antigen, membrane attack complex (MAC) inhibition factor, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, MGC2354, MIC11MSK21, MIN1, MIN2, MIN3, MIRL, p18-20, protectin, surface anitgen recognized by monoclonal 16.3A5, T cell-activating protein
• Gene Symbols: CD59
• Isotype: IgM κ
• Purification Method: Protein A or G purified
• Test Specificity: Reacts with human CD59, a 20kDa glycosyl phosphatidyl-inositol (GPI)-anchored cell surface protein (Workshop VI; Code N-L036). CD59 regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. CD59 is widely distributed on cells in all tissues. It inhibits formation of MAC, thus protecting cells from complement-mediated lysis. The expression of CD59 on erythrocytes is important for their survival. Genetic defects in GPI-anchor attachment, that cause a reduction or loss of CD59 and CD55 on erythrocytes produce the symptoms of the disease paroxysmal hemoglobinuria (PNH). This MAb recognizes CD59 transfected cells. It is useful for study on GPI-anchored proteins, PNH and CD5

For Research Use Only