CDC27, Mouse, Clone: 5C12, Abnova™

Mouse monoclonal antibody raised against partial recombinant CDC27.

Supplier:  Abnova Corporation MAB10545

Catalog No. 89-108-734

Description

The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eucaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq

Specifications

• Antigen: CDC27
• Classification: Monoclonal
• Conjugate: Unconjugated
• Dilution: ELISA (1:10000) Western Blot (1:500-1:2000) Immunohistochemistry (1:200-1:1000) The optimal working dilution should be determined by the end user.
• Gene: CDC27
• Gene Symbols: CDC27
• Immunogen: Recombinant protein corresponding to human CDC27.
• Regulatory Status: RUO
• Gene ID (Entrez): 996
• Content And Storage: Store at 4°C. For long term storage store at -20°C.
  Aliquot to avoid repeated freezing and thawing.
• Isotype: IgG1

• Applications: ELISA, Immunohistochemistry, Western Blot
• Clone: 5C12
• Description: Mouse monoclonal antibody raised against partial recombinant CDC27.
• Formulation: In ascites (0.03% sodium azide)
• Gene Alias: APC3/CDC27Hs/D0S1430E/D17S978E/HNUC
• Host Species: Mouse
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Form: Liquid