CDC27, Mouse, Clone: 5C12, Abnova™

Catalog No.	Quantity
89-108-734	100 μL

Catalog No. 89-108-734 Supplier Abnova Corporation Supplier No. MAB10545

Description

Mouse monoclonal antibody raised against partial recombinant CDC27.

The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eucaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq

Specifications

• Antigen: CDC27
• Applications: ELISA, Immunohistochemistry, Western Blot
• Classification: Monoclonal
• Clone: 5C12
• Conjugate: Unconjugated
• Description: Mouse monoclonal antibody raised against partial recombinant CDC27.
• Dilution: ELISA (1:10000) Western Blot (1:500-1:2000) Immunohistochemistry (1:200-1:1000) The optimal working dilution should be determined by the end user.
• Formulation: In ascites (0.03% sodium azide)
• Gene: CDC27
• Gene Alias: APC3/CDC27Hs/D0S1430E/D17S978E/HNUC
• Gene Symbols: CDC27
• Host Species: Mouse
• Immunogen: Recombinant protein corresponding to human CDC27.
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 996
• Target Species: Human
• Content And Storage: Store at 4°C. For long term storage store at -20°C.
  Aliquot to avoid repeated freezing and thawing.
• Form: Liquid
• Isotype: IgG1