CDC27, Mouse, Clone: AF3.1, Abnova™

Mouse monoclonal antibody raised against synthetic peptide of CDC27.

Supplier:  Abnova Corporation MAB7304

Catalog No. 89-113-161

Description

The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eucaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq

Specifications

• Antigen: CDC27
• Classification: Monoclonal
• Conjugate: Unconjugated
• Dilution: Western Blot (1-2 ug/mL) The optimal working dilution should be determined by the end user.
• Gene: CDC27
• Gene Symbols: CDC27
• Immunogen: A synthetic peptide (conjugated with KLH) corresponding to amino acids 814-823 of human CDC27.
• Regulatory Status: RUO
• Gene ID (Entrez): 996
• Target Species: Bovine, Canine, Human, Mouse, Rat, Xenopus species
• Form: Liquid

• Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
• Clone: AF3.1
• Description: Mouse monoclonal antibody raised against synthetic peptide of CDC27.
• Formulation: In 10mM PBS, pH 7.4
• Gene Alias: APC3/CDC27Hs/D0S1430E/D17S978E/HNUC
• Host Species: Mouse
• Quantity: 100 μg
• Primary or Secondary: Primary
• Test Specificity: Cdc27 (AF3.1). In WB detects a band of approximately 97 KDa.
• Content And Storage: Store at 4°C for short term. For long term storage store at -20°C.
  Aliquot to avoid repeated freezing and thawing.
• Isotype: IgG2a