Mouse anti-CEA, Clone: CEA31, Azide Free, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2345940.2 MG

Catalog No. NBP23459402

Description

CEACAM5/CD66e Monoclonal antibody specifically detects CEACAM5/CD66e in Human, Primate samples. It is validated for Western Blot, Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, CyTOF-ready.

Specifications

• Antigen: CEACAM5/CD66e
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Dilution: Western Blot 0.25-0.5ug/ml, Flow Cytometry 0.5-1ug/million cells, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug protein, Immunohistochemistry-Paraffin 1-2ug/ml, Immunohistochemistry-Frozen 1-2ug/ml, CyTOF-ready
• Gene Accession No.: P06731
• Gene Symbols: CEACAM5
• Immunogen: Human colon carcinoma extract
• Quantity: 0.2 mg
• Primary or Secondary: Primary
• Test Specificity: This antibody recognizes proteins of 80-200kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This MAb does not react with nonspecific cross-reacting antigen (NCA) and with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). Anti-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix
• Target Species: Human, Primate
• Form: Purified

• Applications: Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
• Clone: CEA31
• Conjugate: Unconjugated
• Formulation: 10mM PBS - BSA free with No Preservative
• Gene Alias: Carcinoembryonic antigen, carcinoembryonic antigen-related cell adhesion molecule 5, CD66e antigen, CEACD66e, DKFZp781M2392, Meconium antigen 100
• Host Species: Mouse
• Purification Method: Protein A purified
• Regulatory Status: RUO
• Gene ID (Entrez): 1048
• Reconstitution: Protein A purified
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG1 κ

For Research Use Only