MilliporeSigma™ Mouse anti-CEBPB, Clone: 3H9,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ MABF769

Catalog No. MABF769MI

Description

Specifically detects CEBPB clone: 3H9 in Human samples, and it is validated for ELISA, Western Blotting

The transcription factor CCAAT/Enhancer Binding Protein beta (C/EBPβ, also known as NFIL6) is a highly conserved B-Zip family member. C/EBPβ regulates multiple genes involved in hepatic function, immune function and adipogenesis, and its functions are generally distinct from those of other C/EBP family members (alpha, gamma, delta, epsilon). C/EBPβ is present in cells as three alternate translation products: two transcription activating forms known as LAP1 and LAP2 (∽35 and 32kDa in rodents, ∽43kDa and 40kDa in humans) and a truncated inhibitory form known as LIP (∽20kDa) which lacks the N-terminal transcription activation domain. C/EBP β is phosphorylated at multiple sites which are determinants of some of its activities (e.g. activation of c-fos, adipogenesis). A recent report shows that it is acetylated at Lys215/216, and others report that it can be sumoylated. C/EBPβ is also acetylated at Lys39 in the transcription activation domain, and mutation of Lys39 impairs its ability to activate transcription of multiple genes, including several involved in adipogenesis.

Specifications

• Antigen: CEBPB
• Classification: Monoclonal
• Formulation: Mouse monoclonal IgG1 ascitic fluid containing 0.03% sodium azide.
• Gene Symbols: CEBPB; LAP; TCF5; PP9092
• Immunogen: Purified recombinant fragment of human CEBPB expressed in E. coli.
• Quantity: 100 μL
• Research Discipline: Inflammation and Immunology
• Target Species: Human
• Form: Ascites

• Applications: ELISA, Western Blot
• Clone: 3H9
• Gene Accession No.: P17676
• Host Species: Mouse
• Purification Method: Unpurified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Stable for one year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles which may damage IgG and affect product performance.
• Isotype: IgG1

For Research Use Only