Mouse anti-Cyclin A2, Clone: E67, Azide Free, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2346130.2 MG

Catalog No. NBP23461302

Description

Cyclin A2 Monoclonal antibody specifically detects Cyclin A2 in Human, Mouse, Bovine, Mustelid, Primate samples. It is validated for Western Blot, Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, CyTOF-ready.

Specifications

• Antigen: Cyclin A2
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Dilution: Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug protein, Immunohistochemistry-Paraffin 1-2ug/ml, Immunohistochemistry-Frozen 1-2ug/ml, CyTOF-ready
• Gene Accession No.: P20248
• Gene Symbols: CCNA2
• Immunogen: Full length bovine Cyclin A protein
• Purification Method: Protein A purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: It recognizes a protein of 54kDa, which is identified as cyclin A. Its epitope is located amino acids 144-148 of human Cyclin A2. Cyclins are regulatory subunits of the cyclin-dependent kinases (cdks) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdks. These cyclin/cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, cyclin A is required for S-phase and passage through G2-phase. The D and E type cyclins regulate the passage of G1, while cyclin B is a critical regulator of mitosis. Mutation or disruption of normal cyclin A expression causes cells to arrest in G2-phase.
• Target Species: Human, Mouse, Bovine, Mustelid, Primate
• Form: Purified

• Applications: Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
• Clone: E67
• Conjugate: Unconjugated
• Formulation: 10mM PBS - BSA free with No Preservative
• Gene Alias: CCN1CCNAcyclin-A, cyclin A2, Cyclin-A, cyclin-A2
• Host Species: Mouse
• Molecular Weight of Antigen: 54 kDa
• Quantity: 0.2 mg
• Research Discipline: Cancer, Cell Biology, Cell Cycle and Replication, Cellular Markers, Core ESC Like Genes, Stem Cell Markers, Tumor Suppressors
• Gene ID (Entrez): 890
• Reconstitution: Protein A purified
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG2a κ

For Research Use Only