Cytokeratin 10/13 Antibody (DE-K13), Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2452560.02MG

Catalog No. NBP24525600

Description

Ensure accurate, reproducible results in Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Cytokeratin 10/13 Monoclonal specifically detects Cytokeratin 10/13 in Human, Feline samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence.

Specifications

• Antigen: Cytokeratin 10/13
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Dilution: Western Blot 0.25 - 0.5 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, SDS-Page, Immunofluorescence 0.5 - 1.0 ug/ml
• Gene Alias: BCIE, BIE, CK10, CK-10, cytokeratin 10, Cytokeratin-10, EHK, K10keratosis palmaris et plantaris, keratin 10, keratin, type I cytoskeletal 10, keratin-10, KPP
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This antibody recognizes cytokeratin 10 (56.5kDa) and cytokeratin 13 (53kDa) in Western blotting. It recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on frozen sections this MAb serves as differentiation-related marker of all stratified epithelia; it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas
• Content And Storage: Store at 4C.
• Isotype: IgG2a κ

• Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), SDS-Page, Immunofluorescence
• Clone: DE-K13
• Conjugate: Unconjugated
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Symbols: KRT10
• Immunogen: Cytoskeletal preparation extracted from human ectocervical epithelium.
• Quantity: 0.02 mg
• Research Discipline: Cytoskeleton Markers
• Gene ID (Entrez): 3858
• Target Species: Human, Feline
• Form: Purified

For Research Use Only