Cytokeratin 19 Antibody (A53-B/A2.26), Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2151860.1MG

Catalog No. NBP21518601

Description

Cytokeratin 19 Monoclonal specifically detects Cytokeratin 19 in Human samples. It is validated for Western Blot, Simple Western, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen.

Specifications

• Antigen: Cytokeratin 19
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Dilution: Western Blot 0.5-1ug/ml, Simple Western 10 ug/ml, Flow Cytometry 0.5-1ug/million cells, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml
• Gene Accession No.: P08727
• Gene Symbols: KRT19
• Immunogen: Human breast cancer MCF-7 cells were used as the immunogen for the Keratin-19 antibody.
• Quantity: 0.1 mg
• Research Discipline: Cancer, Cell Biology, Cellular Markers, Cytoskeleton Markers, Neuroscience, Stem Cell Markers
• Gene ID (Entrez): 3880
• Target Species: Human
• Form: Purified

• Applications: Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Clone: A53-B/A2.26
• Conjugate: Unconjugated
• Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
• Gene Alias: CK19, CK-19, cytokeratin 19, Cytokeratin-19,40-kDa keratin intermediate filament, K19cytokeratin-19, K1CS, keratin 19, keratin, type I cytoskeletal 19, keratin, type I, 40-kd, keratin-19, MGC15366
• Host Species: Mouse
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This MAb reacts with the rod domain of human cytokeratin-19 (CK19), a polypeptide of 40kDa. Its epitope maps between amino acid 312-335. CK19 is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50%-60% of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.
• Content And Storage: Store at 4C.
• Isotype: IgG2a κ

For Research Use Only