MilliporeSigma™ Mouse anti-Cytokeratin 5,6, Clone: D5/16B4, Alexa Fluor 555,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ MAB1620AF555

Catalog No. MAB1620AF55

Description

Specifically detects Cytokeratin 5,6 clone: D5/16B4 in Human, Mouse, Rat samples, and it is validated for Immunocytochemistry

Keratin, type II cytoskeletal 5 (UniProt P13647; also known as 58kDa cytokeratin, CK-5, Cytokeratin-5, K5, Keratin-5, Type-II keratin Kb5) is encoded by the KRT5 (also known as DDD1, DM-EBS, EBSMCE, K-EBS, MP-EBS, WC-EBS) gene (Gene ID 3852) in human. Cytokeratin-5 (Keratin-5 or K5) belongs to a family of highly conserved epithelial-specific intermediate filament (IF) proteins. Each epithelium is characterized by its unique keratin expression pattern. In interfollicular epidermis, four major keratins are synthesized and co-polymerize in pairs, K5/K14 and K1/KI0, to form filaments. The expression of these two pairs is strictly regulated during normal keratinocyte differentiation. The K5/K14 filament is expressed in proliferating basal cells, while the K1/K10 filament is expressed during the transition to terminal differentiation and at the cessation of cell division. Similar to other IF members, Keratin-5 contains a central rod region composed of alpha helix segments (a.a. 168-203, 223-315, 339-477) that allow two different IF proteins, one type I (acidic) and one type II (basic) keratin, to intertwine into a coiled-coil formation via hydrophobic interaction. Kearain-5 is a type II keratin that pairs with the type I keratin-14 to form IF.

Specifications

• Antigen: Cytokeratin 5,6
• Classification: Monoclonal
• Conjugate: Alexa Fluor 555
• Gene Accession No.: P13647
• Host Species: Mouse
• Purification Method: Protein A Purfied
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Form: Purified

• Applications: Immunocytochemistry
• Clone: D5/16B4
• Formulation: Purified mouse monoclonal IgG1 antibody conjugate in PBS with 15mg/mL BSA and 0.1% sodium azide.
• Gene Symbols: KRT5; DDD1; DM-EBS; EBSMCE; K-EBS; MP-EBS; WC-EBS
• Immunogen: Purified human cytokeratin 5 (Mischke, D., and Wild, G.A. (1987). J. Invest. Dermatol. 88(2):191-197; Wild, G.A., and Mischke, D. (1986). Exp. Cell Res. 162(1):114-126).
• Quantity: 100 μL
• Research Discipline: Cell Structure
• Gene ID (Entrez): NM_000424.3
• Content And Storage: Stable for one year at 2°C to 8°C from date of receipt.
• Isotype: IgG1

For Research Use Only