Mouse anti-Cytokeratin 8, Clone: KRT8/899, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2449410.2MG

Catalog No. NBP24494102

Description

Ensure accurate, reproducible results in Simple Western, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Cytokeratin 8 Monoclonal antibody specifically detects Cytokeratin 8 in Human, Monkey, Rabbit samples. It is validated for Simple Western, Flow Cytometry, Immunohistochemistry-Paraffin, Immunofluorescence.

Specifications

• Antigen: Cytokeratin 8
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Dilution: Simple Western 10 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 1 - 2 ug/ml
• Gene Alias: CARD2, CK8, CK-8, CYK8cytokeratin 8, Cytokeratin-8, K2C8, K8cytokeratin-8, keratin 8, keratin, type II cytoskeletal 8, keratin-8, KO, Type-II keratin Kb8
• Host Species: Mouse
• Molecular Weight of Antigen: 52.5 kDa
• Quantity: 0.2 mg
• Research Discipline: Cancer, Cytoskeleton Markers
• Gene ID (Entrez): 3856
• Target Species: Human, Monkey, Rabbit
• Form: Purified

• Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
• Clone: KRT8/899
• Conjugate: Unconjugated
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Symbols: KRT8
• Immunogen: Recombinant human KRT8 protein
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). CK8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin 8 and 18. CK8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. Anti-CK8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against CK8, CK18 and CK19). Reportedly, anti-CK8 is useful for the differentiation of lobular (ring-like, perinuclear) from ductal (peripheral-predominant) carcinoma
• Content And Storage: Store at 4C.
• Isotype: IgM κ

For Research Use Only