Mouse anti-Cytokeratin, LMW, Clone: AE1, Azide Free, Novus Biologicals™

Supplier:  Novus Biologicals NBP2331780.2MG

Catalog No. NBP23317802

Specifications

• Antigen: Cytokeratin, LMW
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Dilution: Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml, CyTOF-ready
• Gene Alias: Cytokeratin LMW, cytokeratin-1B, keratin 77, keratin, type II cytoskeletal 1b, KRT77, type-II keratin Kb39
• Host Species: Mouse
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Gene ID (Entrez): 374454
• Target Species: Human, Mouse, Rat, Bovine, Canine, Chicken, Other, Primate, Rabbit
• Form: Purified

• Applications: Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
• Clone: AE1
• Conjugate: Unconjugated
• Formulation: 10mM PBS - BSA free with No Preservative
• Gene Symbols: KRT77
• Immunogen: Human epidermal keratin was used as the immunogen for the Cytokeratin LMW AE1 antibody.
• Quantity: 0.2 mg
• Primary or Secondary: Primary
• Test Specificity: This MAb recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI less than 5.7) and basic (pI less than 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG1 κ

For Research Use Only