Mouse anti-EGF R/ErbB1, Clone: B1D8, Azide Free, Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP23455302	Novus Biologicals NBP2345530.2 MG	0.2 mg

Description

EGFR Monoclonal antibody specifically detects EGFR in Human, Mouse (Negative), Rat (Negative) samples. It is validated for Flow Cytometry, Immunoprecipitation, CyTOF-ready, Block/Neutralize.

Specifications

• Antigen: EGFR
• Concentration: 1.0 mg/mL
• Applications: Flow Cytometry, Immunoprecipitation, Blocking Assay, CyTOF
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Cancer, Cell Biology, Cell Cycle and Replication, Growth and Development, Hypoxia, Phospho Specific, Signal Transduction, Tumor Suppressors, Tyrosine Kinases
• Formulation: 10mM PBS - BSA free with No Preservative
• Gene Alias: avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, cell growth inhibiting protein 40, cell proliferation-inducing protein 61, EC 2.7.10, EC 2.7.10.1, epidermal growth factor receptor, epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog), ERBB, ErbB1, ERBB1PIG61, HER1, mENA, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1
• Gene Symbols: EGFR
• Isotype: IgG2a κ
• Purification Method: Protein A purified
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

• Clone: B1D8
• Dilution: Flow Cytometry 0.5-1ug/million cells, Immunoprecipitation 1-2ug/500ug protein lysate, Blocking/Neutralizing, CyTOF-ready
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Mouse (Negative), Rat (Negative)
• Gene Accession No.: P00533
• Gene ID (Entrez): 1956
• Immunogen: Microsomes from A431 cells
• Primary or Secondary: Primary
• Reconstitution: Protein A purified
• Test Specificity: This antibody reacts with the extracellular domain of EGFR and blocks the EGF/TGF??induced activation. It also blocks tumor growth in vivo. It is excellent for purification of EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, Beta cellulin and epiregulin.

For Research Use Only