MilliporeSigma™ Mouse anti-Ezrin (Ascites Free), Clone: 4A5,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ MAB3822C

Catalog No. MAB3822C

Description

Specifically detects Ezrin (Ascites Free) clone: 4A5 in Human, Mouse, Rabbit, Rat samples, and it is validated for Immunohistochemistry, Immunoprecipitation, Western Blotting

Ezrin (UniProt Q8HZQ5; also known as Cytovillin, Villin-2, p81) is encoded by the EZR (also known as VIL2) gene (Gene ID 100008846) in Oryctolagus cuniculus (Rabbit) species. Ezrin, radixin, and moesin are the three ERM protein family members that crosslink actin filaments with plasma membranes and play an important role in the cell cortex architecture. In its inactive state, ezrin is located in the cytosol in a conformation involving intramolecular interaction between its N- and C-terminal. Ezrin is activated by phosphorylation, which causes a conformation change that disrupts the head-to-tail intramolecular interaction. Upon activation, ezrin binds to the cytoplasmic tails of multiple adhesion and signaling membrane molecules, such as CD44, growth factor receptors, and vascular adhesion molecule (VCAM1). The initiator methionine of ezrin is removed posttranslationally to yield the mature protein (a.a. 2-586) with an N-termina FERM domain (a.a. 2-295) that mediates interaction with microtubules.

Specifications

• Antigen: Ezrin (Ascites Free)
• Classification: Monoclonal
• Formulation: Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
• Gene Symbols: EZR; VIL2
• Immunogen: Purified Ezrin from rabbit gastric parietal cells.
• Quantity: 100 μg
• Research Discipline: Cell Structure
• Gene ID (Entrez): NP_001075591
• Content And Storage: Stable for one year at 2°C to 8°C from date of receipt.
• Isotype: IgG1 κ

• Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
• Clone: 4A5
• Gene Accession No.: Q8HZQ5
• Host Species: Mouse
• Purification Method: Protein G Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rabbit, Rat
• Form: Purified

For Research Use Only