MilliporeSigma™ Mouse anti-Fox1, Clone: 1D10,

Catalog No.	Quantity
MABE985MI	100 μg

Catalog No. MABE985MI Supplier MilliporeSigma™ Supplier No. MABE985

Description

Mouse Monoclonal Antibody

Specifically detects Fox1 clone: 1D10 in Mouse samples, and it is validated for Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blotting

RNA binding protein fox-1 homolog 1 (UniProt Q9JJ43; also known as Ataxin-2-binding protein 1, Fox-1 homolog A, Hexaribonucleotide binding protein 1) is encoded by the Rbfox1 (also known as A2bp, A2bp1, Fox-1, Hrnbp1) gene (ORF MNCb-3035; Gene ID 268859) in murine species. The RNA-binding Fox (Rbfox) family of splicing factors is comprised of three members, Rbfox1 (Fox-1 or A2BP1), Rbfox2 (Fox-2 or RBM9), and Rbfox3 (Fox-3, HRNBP3 or NeuN). Rbfox proteins regulate splicing of many neuronal transcripts (pre-mRNAs) by binding the sequence (U)GCAUG in introns flanking alternative exons. A (U)GCAUG motif that lies downstream of the alternative exon generally promotes Rbfox-dependent exon inclusion, whereas an upstream motif will usually repress exon inclusion. Rbfox1 transcript itself is subjected to alternative splicing in response to chronic cell depolarization. This results in the increased expression of the nuclear, splicing-active Rbfox1 isoform, which in turn leads to increased splicing of Rbfox1 target transcripts of proteins, including N-methyl D-aspartate (NMDA) receptor 1 (Grin1) and a calcium ATPase (Atp2b1), that play important roles in neuronal excitation and calcium homeostasis.

Specifications

• Antigen: Fox1
• Applications: Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
• Classification: Monoclonal
• Clone: 1D10
• Formulation: Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
• Gene Accession No.: Q9JJ43
• Gene Symbols: Rbfox1; A2bp; A2bp1; Fox1; MNCb-3035
• Host Species: Mouse
• Immunogen: GST-tagged recombinant protein corresponding to mouse Fox1.
• Purification Method: Protein G Purified
• Quantity: 100 μg
• Regulatory Status: RUO
• Research Discipline: Epigenetics and Nuclear Function
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_067452
• Target Species: Mouse
• Content And Storage: Stable for one year at 2°C to 8°C from date of receipt.
• Form: Purified
• Isotype: IgG2a κ

For Research Use Only