MilliporeSigma™ Mouse anti-gamma-Actin/ACTG1, Clone: 2A3,

Catalog No.	Quantity
MABT824	100 μg

Catalog No. MABT824 Supplier MilliporeSigma™ Supplier No. MABT824

Description

Mouse Monoclonal Antibody

Specifically detects gamma-Actin/ACTG1 clone: 2A3 in Chicken, Human, Mouse, Rat samples, and it is validated for Immunocytochemistry, Western Blotting

Actin, cytoplasmic 2 (UniProt P63261; also known as Gamma-actin) is encoded by the ACTG1 (also known as ACTG, BRWS2, DFNA20, DFNA26) gene (Gene ID 71) in human. Actins are globular multi-functional proteins that serve as the basic building blocks of cytoskeletal microfilaments and are among the most conserved eukaryotic proteins. Six actin types exist, skeletal muscle alpha-actin is encoded by the ACTA1 gene, smooth muscle alpha-actin by the ACTA2 gene, cytoplasmic beta-actin by the ACTB gene, cardiac muscle alpha-actin by the ACTC1 gene, cytoplasmic gamma-actin by the ACTG1 gene, and smooth muscle gamma-actin by the ACTG2 (a.k.a. ACTA3) gene. Although actins show 90% overall sequence homology, isoforms do show spatial, temporal, and tissue-specific expression patterns and only 50-60% homology is found in their 18 N-terminal residues. Cytoplasmic β and γ-actins, are thought to be present in all cells, while the other four actin isoforms are typically found in specific adult muscle tissue types. Actins exist in a variety of structural states, depending on the specific ionic conditions or the interaction with ligand proteins. The oligomeric and polymeric forms that actin molecules assume are dependent on the distinct conformations they adopt.

Specifications

• Antigen: gamma-Actin/ACTG1
• Applications: Immunocytochemistry, Western Blot
• Classification: Monoclonal
• Clone: 2A3
• Formulation: Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
• Gene Accession No.: P63261
• Gene Symbols: ACTG1; ACTG; BRWS2; DFNA20; DFNA26
• Host Species: Mouse
• Immunogen: KLH-conjugated linear peptide corresponding to the the N-terminal sequence of human gamma-Actin/ACTG1.
• Purification Method: Protein G Purified
• Quantity: 100 μg
• Regulatory Status: RUO
• Research Discipline: Cell Structure
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_001605.1
• Target Species: Chicken, Human, Mouse, Rat
• Content And Storage: Stable for one year at 2°C to 8°C from date of receipt.
• Form: Purified
• Isotype: IgG2b κ

For Research Use Only