GATA3 Mouse, Brilliant Violet 711, Clone: L50-823, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 565449

Catalog No. BDB565449

Description

GATA3 (GATA binding protein 3) is a member of the GATA family of transcription factors. This ∽50-kDa nuclear protein regulates the development and subsequent maintenance of multiple tissues. GATA3 is involved in the development of T lymphocytes (regulates T cell receptor subunit gene expression) and the differentiation of mature T cells to become Th2 cells. The expressed levels of normal or mutant GATA3 are also associated with the behaviors of various cancer cells including estrogen receptor-positive breast carcinoma cells.

The L50-823 monoclonal antibody recognizes human and mouse GATA3.

The antibody was conjugated to BD Horizon BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm. BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors. However, the spillover can be corrected through compensation as with any other dye combination.

Specifications

• Antigen: GATA3
• Classification: Monoclonal
• Conjugate: Brilliant Violet 711
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Immunogen: mouse and rat GATA3, Conserved peptide between the trans-activation and DNA-binding domains of human
• Quantity: 50 Tests
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Applications: Flow Cytometry, Immunocytochemistry
• Clone: L50-823
• Description: Gata-3; GATA binding protein 3; GATA-binding factor 3; HDR
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Human, Mouse
• Isotype: IgG1 κ

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov