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Mouse anti-Golgi Glycoprotein 1/GLG1, Clone: GLG1/970, Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer:  Novus BiologicalsSupplier Diversity Partner NBP2443610.2MG

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Catalog No. NBP24436102

This item has been discontinued and is no longer available. Please call customer service for assistance: 1-800-766-7000.


Description

Description

Ensure accurate, reproducible results in Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunocytochemistry, Immunofluorescence

Specifications

Specifications

Golgi Glycoprotein 1/GLG1
GLG1/970
Unconjugated
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Purified
GLG1
Golgi fraction from human liver cells
134 kDa
0.2 mg
Store at 4C.
Monoclonal
This MAb recognizes a protein of 134kDa, which binds fibroblast growth factor and E-selectin (cell-adhesion lectin on endothelial cells mediating the binding of neutrophils). Fucosylation is essential for binding to E-selectin. It contains sialic acid residues and 16 Cys-rich GLG1 repeats. This MAb can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae. The Golgi tends to be more pronounced and numerous in cells that make and secrete many substances such as plasma B cells.
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, Immunocytochemistry
0.2mg/mL
Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml, Immunocytochemistry 0.5 - 1.0 ug/ml
Q92896, Q92896
CFR1, CFR-1, cysteine-rich fibroblast growth factor receptor, DKFZp686L08213, E-selectin ligand 1, ESL1, ESL-1, FLJ23319, FLJ23967, FLJ32797, Golgi apparatus protein 1, golgi glycoprotein 1, golgi sialoglycoprotein MG-160, MG160, MG-160
Mouse
IgG1 Kappa
Protein A or G purified
RUO
Primary
2734
Human, Mouse (Negative), Rat (Negative)
Documents
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