HLA-B, Mouse, Clone: EP-4, Abnova™

Catalog No.	Quantity
89-112-164	100 μg

Catalog No. 89-112-164 Supplier Abnova Corporation Supplier No. MAB5202

Description

Mouse monoclonal antibody raised against native HLA-B.

HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described. [provided by RefSeq

Specifications

• Antigen: HLA-B
• Applications: Flow Cytometry, Immunohistochemistry
• Classification: Monoclonal
• Clone: EP-4
• Conjugate: Unconjugated
• Description: Mouse monoclonal antibody raised against native HLA-B.
• Dilution: The optimal working dilution should be determined by the end user.
• Formulation: In PBS (0.05% sodium azide)
• Gene: HLA-B
• Gene Alias: AS/HLA-B-7301/HLA-B73/HLAB/HLAC/SPDA1
• Gene Symbols: HLA-B
• Host Species: Mouse
• Immunogen: Native purified from lymphocytes of HLA-B27 individual.
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 3106
• Test Specificity: EP-4 recognizes the HLA-B27 cell surface antigen on human cells. Since EP-4 recognizes the HLA-B27 antigen, it may be used to HLA type human lymphoctyes. Approximately 60% of patients with ankylosing spondylitis are HLA-B27 positive. This reagent can be used to help identify this HLA haplotype in human lymphocytes. Monoclonal antibody EP-4 recognizes the HLA-B27 antigen expressed on individuals with this HLA haplotype.
• Target Species: Human
• Content And Storage: Store at 4°C. For long term storage store at -20°C.
  Aliquot to avoid repeated freezing and thawing.
• Form: Liquid
• Isotype: IgM κ