HLA-DRB1, Mouse, Clone: DA2, Abnova™

Catalog No.	Quantity
89-113-151	100 μg

Catalog No. 89-113-151 Supplier Abnova Corporation Supplier No. MAB7239

Description

Mouse monoclonal antibody raised against native HLA-DRB1.

HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28kDa. It is encoded by 6 exons. Exon one encodes the leader peptide; exons 2 and 3 encode the two extracellular domains; exon 4 encodes the transmembrane domain; and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogs DRB3, DRB4 and DRB5. DRB1 is present in all individuals. Allelic variants of DRB1 are linked with either none or one of the genes DRB3, DRB4 and DRB5. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9. [provided by RefSeq

Specifications

• Antigen: HLA-DRB1
• Applications: ELISA, Flow Cytometry, Immunohistochemistry (Frozen), Western Blot
• Classification: Monoclonal
• Clone: DA2
• Conjugate: Unconjugated
• Description: Mouse monoclonal antibody raised against native HLA-DRB1.
• Dilution: The optimal working dilution should be determined by the end user.
• Formulation: In PBS
• Gene: HLA-DRB1
• Gene Alias: DRB1/FLJ76359/HLA-DR1B/HLA-DRB/HLA-DRB1*/SS1
• Gene Symbols: HLA-DRB1
• Host Species: Mouse
• Immunogen: Native purified HLA-DRB1 from the membranes of LKT cells.
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 3123
• Test Specificity: HLA-DR beta chain 1. All DR and DP except DR7. Not specific to humans. WB: Predicted molecular weight: 30 KDa (unprocessed precursor). Epitope is the 1st domain, monomorphic.
• Target Species: Human
• Content And Storage: Store at 4°C for short term. For long term storage store at -20°C.
  Aliquot to avoid repeated freezing and thawing.
• Form: Liquid
• Isotype: IgG1