HLA-G, Mouse, PE, Clone: 5A6G7, Abnova™

Mouse monoclonal antibody raised against synthetic peptide HLA-G.

Supplier:  Abnova Corporation MAB4489

Catalog No. 89-111-856

Description

HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq

Specifications

• Antigen: HLA-G
• Classification: Monoclonal
• Concentration: 0.1 mg/mL
• Description: Mouse monoclonal antibody raised against synthetic peptide HLA-G.
• Formulation: In PBS (0.2% BSA, 0.09% sodium azide)
• Gene Alias: MHC-G
• Host Species: Mouse
• Quantity: 100 μg
• Primary or Secondary: Primary
• Test Specificity: This antibody allows to discriminate between soluble HLA-G protein yielded by shedding from membrane-bound HLA-G forms that do not contain the intron 4-encoded epitope and soluble HLA-G5/HLA-G6 produced from intron 4-retaining alternatively spliced mRNAs. HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells.
• Content And Storage: Store in the dark at 4°C. Do not freeze.
  Avoid prolonged exposure to light.
  Aliquot to avoid repeated freezing and thawing.
• Isotype: IgG1

• Applications: ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (PFA fixed), Western Blot
• Clone: 5A6G7
• Conjugate: PE
• Dilution: Flow Cytometry (10 ug/mL) The optimal working dilution should be determined by the end user.
• Gene: HLA-G
• Gene Symbols: HLA-G
• Immunogen: A synthetic peptide (conjugated with OVA) corresponding to C-terminus of human soluble HLA-G5 and HLA-G6.
• Regulatory Status: RUO
• Gene ID (Entrez): 3135
• Target Species: Human
• Form: Liquid