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CD13 Mouse anti-Human, Brilliant Violet 711, Clone: WM15, BD Optibuild™
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The WM15 monoclonal antibody specifically binds to CD13, the 150 kDa Type II integral membrane glycoprotein which is also known as aminopeptidase N. This antibody binds to GM-progenitor cells, granulocytic and monocytic cells, and mast cells, but not to lymphocytes, platelets or erythrocytes. Aminopeptidase N is involved in the metabolism of many regulatory peptides.
The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm. BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors. However, the spillover can be corrected through compensation as with any other dye combination.
Specifications
Specifications
| Antigen | CD13 |
| Applications | Flow Cytometry |
| Classification | Monoclonal |
| Clone | WM15 |
| Concentration | 0.2mg/mL |
| Conjugate | Brilliant Violet 711 |
| Description | ANPEP; APN; Aminopeptidase N; Alanyl aminopeptidase; LAP1; PEPN |
| Formulation | Aqueous buffered solution containing ≤0.09% sodium azide. |
| Host Species | Mouse |
| Immunogen | Human AML Cells |
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Safety and Handling
For Research Use Only
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