Learn More
CD15 Mouse anti-Human, Brilliant Violet 421, Clone: W6D3, BD Optibuild™
Mouse Monoclonal Antibody
Supplier: BD Biosciences 740086
Description
The W6D3 monoclonal antibody specifically binds to 3-fucosyl-N-acetyllactosamine (3-FAL), a 220 kDa carbohydrate structure, also called X-hapten. 3-FAL is expressed on >95% of granulocytes, including neutrophils and eosinophils, and on monocytes to a varying degree, but not on lymphocytes or basophils. CD15 plays a role in mediating phagocytosis, bactericidal activity and chemotaxis. Most CD15 antibodies are IgM isotype; clone W6D3 is a mouse IgG1 isotype. In comparison studies with clone HI98, a known CD15 antibody, clone W6D3 shows brighter fluorescence staining and its binding can be blocked by clone HI98.
The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.
Specifications
CD15 | |
Monoclonal | |
0.2mg/mL | |
3-fucosyl-N-acetyllactosamine; 3-FAL | |
Mouse | |
50 μg | |
Primary | |
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
Flow Cytometry | |
W6D3 | |
Brilliant Violet 421 | |
Aqueous buffered solution containing ≤0.09% sodium azide. | |
Affinity Purified | |
RUO | |
Human | |
IgG1 κ |
We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.
For Research Use Only