CD22 Mouse anti-Human, Brilliant Violet 605, Clone: HIB22, BD Optibuild
Mouse Monoclonal Antibody
Manufacturer: BD Biosciences 740396
The HIB22 monoclonal antibody specifically binds to CD22. CD22 is a 130-140 kDa glycosylated type I integral membrane protein present on the surface of mature B cells. CD22 is expressed in the cytoplasm of virtually all B cells except plasma cells. CD45RO antigen on T cells and CD75 antigen on B cells have been identified as ligands for CD22. CD22 has been reported to participate in B-cell activation and also as an adhesion molecule. Although the immunobiology of this antigen has not been fully elucidated, reports indicate that ligation of CD22 induces constitutive internalization of the molecule followed by complete degradation.
This antibody is conjugated to BD Horizon BV605 which is part of the BD Horizon Brilliant Violet family of dyes. With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.
|Brilliant Violet 605|
|Aqueous buffered solution containing ≤0.09% sodium azide.|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|BL-CAM; Siglec-2; Bgp135; Lyb8; LPAP|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
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