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CD23 Mouse anti-Human, Brilliant Violet 650, Clone: M-L233, BD Optibuild™
Mouse Monoclonal Antibody
Supplier: BD Biosciences 740591
Description
The M-L233 antibody specifically binds to human CD23, the low affinity receptor for human IgE (Fc ε RII). CD23 is a type II membrane glycoprotein that can be expressed by B cells, monocytes, macrophages, eosinophils, platelets and dendritic cells. CD23 can mediate IgE-dependent cytotoxicity and phagocytosis by macrophages and eosinophils. Soluble CD23 (sCD23) can be released by CD23-positive cells as a result of proteolytic cleavage of membrane CD23. Larger fragments of sCD23 (e.g., 25-37 kDa) retain their IgE-binding capacity whereas smaller fragments (i.e., ≤ 12 kDa) do not. Soluble CD23 may have immunoregulatory effects on the growth and differentiation of B cells and other cell types.
The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.
Specifications
CD23 | |
Monoclonal | |
0.2mg/mL | |
FCER2; FcεRII; Low affinity immunoglobulin epsilon Fc receptor; BLAST-2 | |
Mouse | |
50 μg | |
Primary | |
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
Flow Cytometry | |
M-L233 | |
Brilliant Violet 650 | |
Aqueous buffered solution containing ≤0.09% sodium azide. | |
Affinity Purified | |
RUO | |
Human | |
IgG1 κ |
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