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CD25 Mouse anti-Human, Brilliant Violet 510, Clone: 2A3, BD Optibuild™

Catalog No. BDB740198 Shop All BD Biosciences Products
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BDB740198 50 μg
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Catalog No. BDB740198 Supplier BD Biosciences Supplier No. 740198
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Description

Mouse Monoclonal Antibody

The 2A3 monoclonal antibody specifically binds to human CD25, the low-affinity alpha subunit of the Interleukin-2 Receptor (IL- 2Rα). CD25 associates with CD122 (IL-2Rβ chain) and CD132 (common γchain or γc) to form the high-affinity signal-transducing IL -2R complex. CD25 is expressed by subsets of thymocytes and peripheral blood lymphocytes including CD4+CD25+ regulatory T cells and memory T cells. CD25 antigen density increases on activated T cells including phytohemagglutinin (PHA)-, concanavalin A (Con A)-, and CD3-activated T lymphocytes. High levels of CD25 can be expressed by T lymphocytes from mixed lymphocyte cultures and by human T-lymphocyte leukemia virus (HTLV)-infected T-lymphocyte leukemia lines, for example, HUT-102. CD25 can also be expressed by activated B cells and macrophages. Recombinant IL-2 blocks the binding of the 2A3 antibody to PHA-activated T lymphocytes.

The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50-nm) filter set. BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser.

TRUSTED_SUSTAINABILITY

Specifications

Antigen CD25
Applications Flow Cytometry
Classification Monoclonal
Clone 2A3
Concentration 0.2mg/mL
Conjugate Brilliant Violet 510
Description IL-2R; IL2RA; IL-2Rα; TCGFR; TAC antigen; p55
Formulation Aqueous buffered solution containing ≤0.09% sodium azide.
Host Species Mouse
Immunogen Human Phytohemagglutinin-activated T Cells
Purification Method Affinity Purified
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Human
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG1 κ
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