CD274 Mouse anti-Human, Brilliant Violet 605, Clone: MIH1, BD Optibuild
Mouse Monoclonal Antibody
Manufacturer: BD Biosciences 740426
PD-L1 and PD-L2 are newly discovered members of the B7 family are the ligands for the program death 1 (PD-1) receptor. They are expressed on immature dendritic cells and mature dendritic cells. PD-L1, also called B7-H1, is expressed on antigen-presenting cells, including IFN- γ-stimulated monocytes, and activated human and murine dendritic cells. Monoclonal antibodies that block PD-L1 and PD-L2 on dendritic cells result in enhanced T cell proliferation and cytokine production. PD-L1 is also expressed on placental trophoblasts, myocardial endothelium, cortical thymic epithelial cells, and on most carcinomas. Studies show overlapping functions of PD-L1 and PD-L2 and indicate an important role for the PD-L:PD-1 pathway in regulating T cell responses.
This antibody is conjugated to BD Horizon BV605 which is part of the BD Horizon Brilliant Violet family of dyes. With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.
|Brilliant Violet 605|
|Aqueous buffered solution containing ≤0.09% sodium azide.|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
For Research Use Only