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CD3 Mouse anti-Human, BUV395, Clone: HIT3a, BD Optibuild™

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 740283

Catalog No. BDB740283


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Description

Description

The HIT3a monoclonal antibody specifically binds to the human CD3 ε-chain, a 20 kDa subunit of the CD3/T cell antigen receptor complex found on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes. Studies from the HLDA Workshop show that this antibody can be mitogenic for T lymphocytes. The CD3 complex plays a role in signal transduction during antigen recognition by the T cell receptor. HIT3a antibody does not stain intracellular CD3 unlike the other CD3-specific clone, UCHT1 (Cat. No. 555330/550368).

The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry. With an Ex Max at 348nm and an Em Max at 395nm, BD Horizon BUV395 can be excited with a 355nm laser and detected with a 379/28 filter.

Specifications

Specifications

CD3
HIT3a
Brilliant Ultraviolet 395
Aqueous buffered solution containing ≤0.09% sodium azide.
Murine
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
RUO
Primary
Human
Flow Cytometry
0.2mg/mL
CD3E; T3E; TCRE; cd 3; cd-3; cd3; CD3-epsilon; 916
Affinity Purified
IgG2a, κ
50μg
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Monoclonal
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