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CD3 Mouse anti-Human, Brilliant Violet 421, Clone: HIT3a, BD Optibuild™
Mouse Monoclonal Antibody
Supplier: BD Biosciences 740073
Description
The HIT3a monoclonal antibody specifically binds to the human CD3 ε-chain, a 20 kDa subunit of the CD3/T cell antigen receptor complex found on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes. Studies from the HLDA Workshop show that this antibody can be mitogenic for T lymphocytes. The CD3 complex plays a role in signal transduction during antigen recognition by the T cell receptor. HIT3a antibody does not stain intracellular CD3 unlike the other CD3-specific clone, UCHT1 (Cat. No. 555330/550368).
The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.
Specifications
CD3 | |
Monoclonal | |
0.2mg/mL | |
CD3E; T3E; TCRE; cd 3; cd-3; cd3; CD3-epsilon; 916 | |
Mouse | |
50 μg | |
Primary | |
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
Flow Cytometry | |
HIT3a | |
Brilliant Violet 421 | |
Aqueous buffered solution containing ≤0.09% sodium azide. | |
Affinity Purified | |
RUO | |
Human | |
IgG2a κ |
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For Research Use Only