CD3 Mouse anti-Human, Brilliant Violet 510, Clone: SP34-2, BD Optibuild™

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 740187

Catalog No. BDB740187

Description

Clone SP34-2 is a mouse IgG1 isotype monoclonal antibody, descendant of SP34 (mouse IgG3), with the same specificity and reactivity pattern as the parent clone. It cross-reacts with a major subset of peripheral blood lymphocytes, but not monocytes or granulocytes, of baboon, and rhesus, cynomolgus, and pigtail macaque monkeys. The distribution on lymphocytes is similar to that observed with normal human donor lymphocytes with the majority of CD3-positive cells being negative when dual stained with antibodies to B or NK cells markers. SP34-2 is also capable of inducing cell proliferation on both human and non-human primate PBMC.

The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50-nm) filter set. BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser.

Specifications

• Antigen: CD3
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Description: CD3E; CD3-epsilon; T3E; TCRE
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Baboon, Cynomolgus, Human, Rhesus Monkey
• Isotype: IgG1 λ

• Applications: Flow Cytometry
• Clone: SP34-2
• Conjugate: Brilliant Violet 510
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: Purified Human CD3ε Protein
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only