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CD3 Mouse anti-Human, Brilliant Violet 711, Clone: SP34-2, BD Optibuild™

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 740807

Catalog No. BDB740807


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Description

Description

Clone SP34-2 is a mouse IgG1 isotype monoclonal antibody, descendant of SP34 (mouse IgG3), with the same specificity and reactivity pattern as the parent clone. It cross-reacts with a major subset of peripheral blood lymphocytes, but not monocytes or granulocytes, of baboon, and rhesus, cynomolgus, and pigtail macaque monkeys. The distribution on lymphocytes is similar to that observed with normal human donor lymphocytes with the majority of CD3-positive cells being negative when dual stained with antibodies to B or NK cells markers. SP34-2 is also capable of inducing cell proliferation on both human and non-human primate PBMC.

The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm. BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors. However, the spillover can be corrected through compensation as with any other dye combination.

Specifications

Specifications

CD3
SP34-2
Brilliant Violet 711
Aqueous buffered solution containing ≤0.09% sodium azide.
Murine
IgG1, λ
50μg
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Monoclonal
Flow Cytometry
0.2mg/mL
CD3E; CD3-epsilon; T3E; TCRE
Affinity Purified
Purified Human CD3ε Protein
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
RUO
Primary
Baboon, Cynomolgus, Human, Rhesus
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