CD54 Mouse anti-Human, Brilliant Violet 786, Clone: HA58, BD Optibuild™

Catalog No.	Quantity
BDB740978	50 μg

Catalog No. BDB740978 Supplier BD Biosciences Supplier No. 740978

Description

Mouse Monoclonal Antibody

The HA58 monoclonal antibody specifically binds to CD54 which is also known as ICAM-1 (intracellular adhesion molecule-1). CD54 is an 85-110 kDa type I transmembrane glycoprotein that belongs to the immunoglobulin supergene family. A soluble form of CD54 can also be found in biological fluids. CD54 is expressed on endothelial cells and both resting (weak) and activated (moderate) lymphocytes and monocytes. CD54 is a ligand for LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). The CD54 adhesion molecule plays roles in inflammatory and immune responses and neoplasia. This antibody (NA/LE format) blocks the mixed-lymphocyte reaction (MLR) and the purified format is suitable for staining acetone-fixed, frozen tissue sections.

The antibody was conjugated to BD Horizon™ BV786 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 786-nm. BD Horizon BV786 can be excited by the violet laser and detected in a filter used to detect Cy™7-like dyes (eg, 780/60-nm filter).

Specifications

• Antigen: CD54
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: HA58
• Concentration: 0.2mg/mL
• Conjugate: Brilliant Violet 786
• Description: ICAM-1; intercellular adhesion molecule 1; BB2; P3.58
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Host Species: Mouse
• Immunogen: IFN-γ-treated BM314 colonic carcinoma cells
• Purification Method: Affinity Purified
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG1 κ

For Research Use Only