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CD56 Mouse anti-Human, Brilliant Violet 650, Clone: B159, BD Optibuild™
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The B159 monoclonal antibody specifically binds to CD56. CD56 is a heavily glycosylated adhesion protein that is present on a subpopulation of peripheral blood large granular lymphocytes that demonstrate natural killer activity. CD56 is also expressed on a subset of T cells but is not expressed on myeloid cells, erythrocytes or B cells. This antigen is a pan-NK-cell marker. CD56 is virtually identical to an isoform of the neural cell adhesion molecule (NCAM), a structure mediating homotypic and heterotypic cell-cell interactions.
The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.
Specifications
Specifications
| Antigen | CD56 |
| Applications | Flow Cytometry |
| Classification | Monoclonal |
| Clone | B159 |
| Concentration | 0.2mg/mL |
| Conjugate | Brilliant Violet 650 |
| Description | NCAM1; NCAM-1; Neural cell adhesion molecule 1; NCAM; NKH1; MSK39 |
| Formulation | Aqueous buffered solution containing ≤0.09% sodium azide. |
| Host Species | Mouse |
| Immunogen | Human NK Cells |
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Safety and Handling
For Research Use Only
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