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CD56 Mouse anti-Human, Brilliant Violet 711, Clone: B159, BD Optibuild™
Mouse Monoclonal Antibody
Supplier: BD Biosciences 740781
Description
The B159 monoclonal antibody specifically binds to CD56. CD56 is a heavily glycosylated adhesion protein that is present on a subpopulation of peripheral blood large granular lymphocytes that demonstrate natural killer activity. CD56 is also expressed on a subset of T cells but is not expressed on myeloid cells, erythrocytes or B cells. This antigen is a pan-NK-cell marker. CD56 is virtually identical to an isoform of the neural cell adhesion molecule (NCAM), a structure mediating homotypic and heterotypic cell-cell interactions.
The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm. BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors. However, the spillover can be corrected through compensation as with any other dye combination.

Specifications
CD56 | |
Monoclonal | |
0.2mg/mL | |
NCAM1; NCAM-1; Neural cell adhesion molecule 1; NCAM; NKH1; MSK39 | |
Mouse | |
Affinity Purified | |
RUO | |
Human | |
IgG1 κ |
Flow Cytometry | |
B159 | |
Brilliant Violet 711 | |
Aqueous buffered solution containing ≤0.09% sodium azide. | |
Human NK Cells | |
50 μg | |
Primary | |
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
Safety and Handling
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For Research Use Only