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Mouse anti-Insulin, Clone: E2-E3, Azide Free, Novus Biologicals™
SDP

Mouse Monoclonal Antibody

Specifications

Antigen Insulin
Clone E2-E3 (same as INS04)
Concentration 1.0 mg/mL
Dilution Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml, CyTOF-ready
Applications Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
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Products 1
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
NBP23461102
SDP
View Documents
Novus Biologicals
NBP2346110.2 MG
0.2 mg N/A N/A N/A
Description

Description

Insulin Monoclonal antibody specifically detects Insulin in Human, Mouse, Porcine, Bovine, Rabbit samples. It is validated for Western Blot, Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, CyTOF-ready.
Specifications

Specifications

Insulin
1.0 mg/mL
Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Unconjugated
Mouse
Diabetes Research, Immune System Diseases, Immunology, Stem Cell Markers
10mM PBS - BSA free with No Preservative
IDDM2, ILPR, insulin, IRDN, MODY10, proinsulin
INS
IgG1 κ
Protein A purified
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
6 kDa
E2-E3 (same as INS04)
Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml, CyTOF-ready
Monoclonal
Purified
RUO
Human, Mouse, Pig, Bovine, Rabbit
P01308
3630
Full length (1-84 amino acid) purified pig insulin, conjugated to KLH
Primary
Protein A purified
Recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as beta-cell and insulinoma marker.
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