Mouse anti-Kappa Light Chain, Clone: KLC709, Azide Free, Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP23466002	Novus Biologicals NBP2346600.2 MG	0.2 mg

Description

Kappa Light Chain Monoclonal antibody specifically detects Kappa Light Chain in Human samples. It is validated for Flow Cytometry, ELISA, Immunohistochemistry-Frozen, CyTOF-ready.

Specifications

• Antigen: Kappa Light Chain
• Concentration: 1.0 mg/mL
• Applications: Flow Cytometry, ELISA, Immunohistochemistry (Frozen), CyTOF
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Adaptive Immunity, Immunology
• Formulation: 10mM PBS - BSA free with No Preservative
• Gene Alias: HCAK1, IGKCD, immunoglobulin kappa constant, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809
• Gene Symbols: IGKC
• Isotype: IgG1 κ
• Purification Method: Protein A purified
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Molecular Weight of Antigen: 22.5 kDa

• Clone: KLC709
• Dilution: Flow Cytometry 0.5-1ug/million cells, ELISA 1-5ug/ml for coating, Immunohistochemistry-Frozen 0.5-1ug/ml, CyTOF-ready
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human
• Gene Accession No.: P01601
• Gene ID (Entrez): 3514
• Immunogen: Recombinant human Ig kappa chain
• Primary or Secondary: Primary
• Reconstitution: Protein A purified
• Test Specificity: This MAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. It recognizes human Ig kappa light chains of both secreted and cell surface immunoglobulin. It detects also free kappa light chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

For Research Use Only