MilliporeSigma™ Mouse anti-Lipoprotein Lipase/LPL, Clone:,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ MABS1270

Catalog No. MABS1270

Description

Specifically detects Lipoprotein Lipase/LPL in Bovine, Human, Mouse, Rat samples, and it is validated for ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blotting

Lipoprotein lipase (EC 3.1.1.34; UniProt P06858; also known as hLPL, LPL) is encoded by the LPL (also known as LIPD) gene (Gene ID 4023) in human. Lipoprotein lipase (LPL) catalyzes the hydrolysis of triglycerides in plasma lipoproteins. LPL is produced by adipocytes and myocytes and secreted into the interstitial spaces, where it is bound by GPIHBP1 (a glycosylphosphatidylinositol-anchored protein of capillary endothelial cells) and shuttled to the luminal face of capillaries. The GPIHBP1–LPL complex is crucial for the binding of triglyceride-rich lipoproteins (TRLs) to endothelial cells and the subsequent lipolytic processing of TRLs. TRLs bind only the LPL-GPIHBP1 complex, but not GPIHBP1 alone, on the cell surface. A deficiency of either protein results in severe hypertriglyceridemia (chylomicronemia) and impaired delivery of lipid nutrients to parenchymal cells. LPL is produced with a signal peptide sequence (a.a. 1-27), the removal of which yields the mature 448-amino acid (a.a. 28-475) enzyme containing a PLAT (Polycystin-1, Lipoxygenase, Alpha-Toxin) domain (a.a. 341-464) and a heparin-binding domain (a.a. 346-441).

Specifications

• Antigen: Lipoprotein Lipase/LPL
• Classification: Monoclonal
• Gene Accession No.: P06858
• Host Species: Mouse
• Purification Method: Protein G Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Bovine, Human, Mouse, Rat
• Form: Purified

• Applications: ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot
• Formulation: Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
• Gene Symbols: LPL; LIPD
• Immunogen: Purified human plasma lipoprotein lipase/LPL.
• Quantity: 100 μg
• Research Discipline: Signaling
• Gene ID (Entrez): NP_000228
• Content And Storage: Stable for one year at 2°C to 8°C from date of receipt.
• Isotype: IgG2a κ

For Research Use Only