Mouse anti-MCAM/CD146, Clone: OJ79c, Azide Free, Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP23470102	Novus Biologicals NBP2347010.2 MG	0.2 mg

Description

MCAM/CD146 Monoclonal antibody specifically detects MCAM/CD146 in Human samples. It is validated for Western Blot, Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Frozen, CyTOF-ready.

Specifications

• Antigen: MCAM/CD146
• Concentration: 1.0 mg/mL
• Applications: Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Frozen)
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Cancer, Mesenchymal Stem Cell Markers, Phospho Specific, Stem Cell Markers
• Formulation: 10mM PBS - BSA free with No Preservative
• Gene Alias: CD146, CD146 antigen, cell surface glycoprotein MUC18, Cell surface glycoprotein P1H12, melanoma adhesion molecule, melanoma cell adhesion moleculeS-endo 1 endothelial-associated antigen, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, MUC18Gicerin
• Gene Symbols: MCAM
• Isotype: IgG1 κ
• Purification Method: Protein A purified
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Molecular Weight of Antigen: 130 kDa

• Clone: OJ79c
• Dilution: Western Blot 0.5-1.0ug/ml, Flow Cytometry 0.5-1ug/million cells, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 0.5-1.0ug/ml, Immunoprecipitation 1-2ug/500ug protein, Immunohistochemistry-Frozen 0.5-1ug/ml, CyTOF-ready
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human
• Gene Accession No.: P43121
• Gene ID (Entrez): 4162
• Immunogen: Recombinant human MUC18 protein
• Primary or Secondary: Primary
• Reconstitution: Protein A purified
• Test Specificity: The human Mel-CAM gene maps to chromosome 11q23 and encodes a trans-membrane glycoprotein, also designated MCAM, MUC 18 or CD146, that belongs to the immunoglobulin superfamily and functions as a Ca2+-independent cell adhesion molecule. Mel-CAM expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. Mel-CAM is found on 80% of advanced primary human melanomas and correlates well with development of metastatic disease.

For Research Use Only