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Mitochondria Antibody (AE-1), Novus Biologicals™
SDP

Catalog No. p-5540871 Shop All R&D Systems Products
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Catalog No. Quantity
NBP24437100 0.02 mg
NBP24437101 0.1 mg
NBP24437102 0.2 mg
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Catalog No. NBP24437100 Supplier Novus Biologicals Supplier No. NBP2443710.02MG
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Description

Mouse Monoclonal Antibody

Ensure accurate, reproducible results in Western Blot, ELISA, Immunohistochemistry (Paraffin), Immunofluorescence

Mitochondria Monoclonal specifically detects Mitochondria in Human, Mouse (Negative), Rat (Negative) samples. It is validated for Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

Antigen Mitochondria
Applications Western Blot, Immunohistochemistry (Paraffin), Immunofluorescence
Classification Monoclonal
Clone AE-1
Concentration 0.2mg/mL
Conjugate Unconjugated
Dilution Western Blot 0.25 - 0.5 ug/ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
Formulation 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Host Species Mouse
Immunogen Semi-purified mitochondrial preparation
Molecular Weight of Antigen 60 kDa
Purification Method Protein A or G purified
Quantity 0.02 mg
Regulatory Status RUO
Research Discipline Cellular Markers, Mitochondrial Markers
Primary or Secondary Primary
Test Specificity This MAb recognizes a 60kDa antigen associated with the mitochondria in human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. It recognizes an antigen associated with the mitochondria in human cells only. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells.
Target Species Human, Mouse (Negative), Rat (Negative)
Content And Storage Store at 4C.
Form Purified
Isotype IgG1 κ
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For Research Use Only

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