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CD212 Mouse anti-Mouse, Brilliant Violet 421, Clone: 114, BD Optibuild™

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 740051

Catalog No. BDB740051

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The 114 monoclonal antibody specifically binds to mouse CD212 (the β 1 subunit of IL-12R β 1), originally termed IL-12R β , of the mouse IL-12 receptor complex. The IL-12R β 1 subunit associates with a β 2 subunit to form a heterodimeric IL-12 receptor complex. Each one of the IL-12R subunits exhibits low affinity for IL-12, but in combination, they bind IL-12 with high affinity. The IL-12R β 1 subunit interacts primarily with IL-12 p40 whereas the IL-12R β β 1 has more recently been described to bind IL-23, a heterodimer formed of the p40 subunit from IL-12, and p19. The cytoplasmic regions of the β 1 and β 2 subunits contain the box1 and box2 motifs found in other cytokine receptors such as gp130, LIFR and G-CSFR. IL-12R β 1 are primarily expressed by activated T cells and NK cells. Experiments with IL-12R β 1 deficient mice have shown that IL-12R β 1 is necessary for mouse T and NK cell responsiveness to IL-12 p75. The 114 antibody was generated by immunizing IL-12R β 1 deficient mice of (129 x BALB/c)F1 background with mouse Ba/F3 cells that were stably transfected with IL-12R β 1

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.



Brilliant Violet 421
Aqueous buffered solution containing ≤0.09% sodium azide.
IgG2a, κ
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Flow Cytometry
Il12rb1; IL-12R-beta-1; IL-12 Receptor β1 chain
Affinity Purified
IL-12Rβ1 Transfected Cell Line
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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