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Ly-51 Mouse anti-Mouse, Brilliant Violet 711, Clone: BP-1, BD Optibuild™
Mouse Monoclonal Antibody
Supplier: BD Biosciences 740691
Description
The BP-1 monoclonal antibody specifically recognizes CD249 which is also known as, 6C3/BP-1, Enpep, or Ly-51. CD249 is a glycoprotein cell-surface differentiation antigen, which was originally identified on pre-B cell lymphomas (spontaneous and chemical- or retrovirus- transformed, in vitro and in vivo ). CD249 is a homodimeric cell-surface glycoprotein with 140-kDa subunits that has been identified to possess aminopeptidase A (APA) activity. The same antigen is expressed at high levels on bone marrow stromal cell lines which support in vitro B lymphopoieses, on thymic dendritic cells and cortical epithelial cells, and on a wide variety of mouse and rat tissues known to possess APA activity. Subsets of normal bone marrow pre-B and B lymphocytes express low levels of CD249 which is rapidly up-regulated on the pre-B cells in the presence of IL-7. A role for the CD249 molecule in the IL-7-driven proliferation of B cell precursors has been postulated. However, B-cell abnormalities were not detected in CD249/Ly-51-deficient mice. Mature B lymphocytes, thymocytes, peripheral T lymphocytes, erythroid cells, and myeloid cells (with the exception of thymic dendritic cells) do not express CD249.
The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm. BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors. However, the spillover can be corrected through compensation as with any other dye combination.
Specifications
Ly-51 | |
Monoclonal | |
0.2mg/mL | |
Bp-1/6C3; Ly51; Ly-51; Enpep; Glutamyl aminopeptidase; APA; EAP | |
Mouse | |
Affinity Purified | |
RUO | |
Mouse | |
IgG2a κ |
Flow Cytometry | |
BP-1 | |
Brilliant Violet 711 | |
Aqueous buffered solution containing ≤0.09% sodium azide. | |
Abelson murine leukemia virus-transformed BALB/c mouse pre-B cell line 18.81 | |
50 μg | |
Primary | |
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
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