MilliporeSigma™ Mouse anti-MPR300/IGF-2R/CD222, Clone: 2C2,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ MABF935

Catalog No. MABF935

Description

Specifically detects MPR300/IGF-2R/CD222 clone: 2C2 in Human samples, and it is validated for Immunocytochemistry, Immunohistochemistry, Western Blotting

Cation-independent mannose-6-phosphate receptor (UniProt P11717; also known as 300kDa mannose 6-phosphate receptor, CD222, CI Man-6-P receptor, CI-MPR, IGF-II receptor, Insulin-like growth factor 2 receptor, Insulin-like growth factor II receptor, M6P/IGF2 receptor, M6P/IGF2R, M6P-R, M6PR, MPR 300) is encoded by the IGF2R (also known as CIMPR, MPRI) gene (Gene ID 3482) in human. Mannose 6-phosphate (M6P) receptors (MPRs) mediate the delivery of newly synthesized, mannose 6-phosphate-bound lysosomal hydrolases from the secretory pathway to the endosomal system. MPRs bind lysosomal hydrolases in the trans-Golgi network (TGN) and unload the enzyme in the endosomal system. There exist a cation-independent CI-MPR (IGF2R) and a smaller CD-MPR that requires divalent cations for lysosomal hydrolases recognition. MPRs also undergo recycling between the endosomal system and the plasma membrane, where CI-MPR mediates the internalization of other ligands, including insulin-like growth factor II (IGF-II). CI-MPR/IGF2R is produced with a signal peptide (a.a. 1-40), the removal of which yields the mature receptor with a large lumenal/extracellular region (a.a. 41-2304), a transmembrane segment (a.a. 2305-2327), and a cytoplasmic domain (a.a. 2328-2491), The extracellular region is composed of 15 P-type carbohydrate recognition domains, also referred to as MRH (mannose 6-phosphate receptor homology) domains. Domains 3 and 9 bind M6P with high affinity and domain 5 binds M6P with weak affinity, while domain 11 binds IGF-II.

Specifications

• Antigen: MPR300/IGF-2R/CD222
• Classification: Monoclonal
• Formulation: Purified mouse monoclonal IgG1κ antibody in PBS without preservatives.
• Gene Symbols: IGF2R; CIMPR; MPRI
• Immunogen: Recombinant full-length human MPR300/IGF-2R/CD222.
• Quantity: 100 μg
• Research Discipline: Inflammation and Immunology
• Gene ID (Entrez): NP_000867
• Content And Storage: Stable for one year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles which may damage IgG and affect product performance.
• Isotype: IgG1 κ

• Applications: Immunocytochemistry, Immunohistochemistry, Western Blot
• Clone: 2C2
• Gene Accession No.: P11717
• Host Species: Mouse
• Purification Method: Protein G Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human
• Form: Purified

For Research Use Only