MUC3 Antibody (MUC3/1154), Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2444340.02MG

Catalog No. NBP24443400

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

MUC3 Monoclonal specifically detects MUC3 in Human, Rat (Negative) samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence.

Specifications

• Antigen: MUC3
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 1 - 2 ug/ml
• Gene Accession No.: Q02505, Q02505, Q9H195
• Gene Symbols: MUC3A
• Immunogen: A synthetic peptide of 35 amino acids, SIB35 (C-HSTPSFTSSITTTETTSHSTPSFTSSITTTETTS), which contains two of the MUC3 tandem repeats, coupled to KLH.
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Gene ID (Entrez): 4584
• Target Species: Human, Rat (Negative)
• Form: Purified

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
• Clone: MUC3/1154
• Conjugate: Unconjugated
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Alias: MUC-3A, mucin 3, intestinal, mucin 3A, cell surface associated, mucin 3A, intestinal, mucin-3A
• Host Species: Mouse
• Molecular Weight of Antigen: 1100 kDa
• Quantity: 0.02 mg
• Primary or Secondary: Primary
• Test Specificity: It recognizes a protein of HMW, identified as mucin 3 glycoprotein (MUC3). This MAb shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC2. The Mucins are a family of highly glycosylated, secreted proteins with a basic structure consisting of a variable number of tandem repeats (VNTRs) encoded by 60 base pairs (Mucin 1), 69 base pairs (Mucin 2) and 51 base pairs (Mucin 3). The number of repeats is highly polymorphic and varies among different alleles. Mucin 1 proteins are expressed as type I membrane proteins in addition to secreted forms. Mucin 1 is aberrantly expressed in epithelial tumors including breast carcinomas. Mucin 2 coats the epithelia of the intestines and airways and is associated with colonic tumors. Mucin 3 is a major component of various mucus gels and is broadly expressed in normal and tumor cells.
• Content And Storage: Store at 4C.
• Isotype: IgG2a κ

For Research Use Only