MilliporeSigma™ Mouse anti-N6-methyladenosine (m6A), Clone: 17-3-4-1,

Catalog No.	Quantity
MABE1006MI	100 μg

Catalog No. MABE1006MI Supplier MilliporeSigma™ Supplier No. MABE1006

Description

Mouse Monoclonal Antibody

Specifically detects N6-methyladenosine (m6A) clone: 17-3-4-1 in All samples, and it is validated for Dot Blot, Immunoprecipitation

N6-Methyladenosine (m6A ) is an abundant modification found in mRNA, tRNA, snRNA, as well as long non-coding RNA, in all species. RNA adenosine methylation is catalyzed by a multicomponent complex composed of METTL3/MT-A70, METTL14, and WTAP in mammals. METTL3 & METTL14 are responsible for the methyltransferase activity of the complex, and WTAP mediates substrate recruitment. Fat mass and obesity-associated (FTO) and ALKBH5 are two identified demethylases that catalyze oxidative demthylation of m6A. Several functions of m6A modification have been proposed, including mRNA decay, splicing regulation, RNA nuclear export and maturation. (PMID 25171402). Anti-N6-methyladenosine (m6A), clone 17-3-4-1, is a mouse monoclonal that specifically detects RNA molecules with m6A modication.

Specifications

• Antigen: N6-methyladenosine (m6A)
• Applications: Dot Blot, Immunoprecipitation
• Classification: Monoclonal
• Clone: 17-3-4-1
• Formulation: Purified mouse monoclonal IgG1λ in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
• Host Species: Mouse
• Immunogen: Hapten N6-methyladenosine-5'-mono-phosphate conjugated to BSA of all N6-methyladenosine (m6A).
• Purification Method: Protein G Purified
• Quantity: 100 μg
• Regulatory Status: RUO
• Research Discipline: Epigenetics and Nuclear Function
• Primary or Secondary: Primary
• Target Species: All species
• Content And Storage: Stable for one year at 2°C to 8°C from date of receipt.
• Form: Purified
• Isotype: IgG1 λ

For Research Use Only