NAPA, Mouse, Clone: 4E4, Abnova™

Catalog No.	Quantity
89-110-642	100 μg

Catalog No. 89-110-642 Supplier Abnova Corporation Supplier No. MAB1807

Description

Mouse monoclonal antibody raised against recombinant NAPA.

The 'SNARE hypothesis' is a model explaining the process of docking and fusion of vesicles to their target membranes. According to this model, membrane proteins from the vesicle (v-SNAREs) and proteins from the target membrane (t-SNAREs) govern the specificity of vesicle targeting and docking through mutual recognition. Once the 2 classes of SNAREs bind to each other, they form a complex that recruits the general elements of the fusion apparatus, namely NSF (N-ethylmaleimide-sensitive factor) and SNAPs (soluble NSF-attachment proteins), to the site of membrane fusion, thereby forming the 20S fusion complex. Alpha- and gamma-SNAP are found in a wide range of tissues and act synergistically in intra-Golgi transport. The sequence of the predicted 295-amino acid human protein encoded by NAPA shares 37%, 60%, and 67% identity with the sequences of yeast, Drosophila, and squid alpha-SNAP, respectively. Platelets contain some of the same proteins, including NSF, p115/TAP, alpha-SNAP, gamma-SNAP, and the t-SNAREs syntaxin-2 and syntaxin-4, that are used in many vesicular transport processes in other cell types. Platelet exocytosis uses a molecular mechanism similar to that used by other secretory cells, such as neurons, although the proteins used by the platelet and their modes of regulation may be quite different. [provided by RefSeq]

Specifications

• Antigen: NAPA
• Applications: Immunoprecipitation, Western Blot
• Classification: Monoclonal
• Clone: 4E4
• Conjugate: Unconjugated
• Description: Mouse monoclonal antibody raised against recombinant NAPA.
• Dilution: Western Blot (0.5-5 ug/mL) Immunoprecipitation (2-10 ug/mL) The optimal working dilution should be determined by the end user.
• Formulation: In 20mM sodium phosphate buffer, 150mM NaCl, pH 7.5 (50% glycerol)
• Gene: NAPA
• Gene Alias: SNAPA
• Gene Symbols: NAPA
• Host Species: Mouse
• Immunogen: Recombinant protein corresponding to human NAPA.
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 8775
• Test Specificity: This antibody specifically recognizes alpha SNAP as a single band of ∼36 KDa on western blot of rat kidney, rat brain and MDBK cells and do not recognize beta SNAP.
• Content And Storage: Store at -20°C.
  Aliquot to avoid repeated freezing and thawing.
• Form: Liquid
• Isotype: IgG1