Mouse anti-NF-H, Clone: NF421 + NFL/736, Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP24528802	Novus Biologicals NBP2452880.2MG	0.2 mg

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

NF-H Monoclonal antibody specifically detects NF-H in Human, Mouse, Rat, Porcine, Chicken samples. It is validated for Flow Cytometry, Immunohistochemistry-Paraffin, Immunofluorescence.

Specifications

• Antigen: NF-H
• Concentration: 0.2mg/mL
• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Autophagy, Cellular Markers, Cytoskeleton Markers, Neurodegeneration, Neurofilaments, Neuronal Cell Markers, Neuroscience
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Alias: 200 kDa neurofilament protein, KIAA0845, Neurofilament Heavy (200kDa), neurofilament heavy polypeptide, Neurofilament triplet H protein, neurofilament, heavy polypeptide, neurofilament, heavy polypeptide 200kDa, NF200, NFH, NF-H
• Gene Symbols: NEFH
• Isotype: IgG
• Purification Method: Protein A or G purified
• Test Specificity: This MAb reacts with a 200kDa and 68kDa protein, identified as heavy and light sub-units of neurofilaments (NF-H & NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.

• Clone: NF421 + NFL/736
• Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml, Immunofluorescence 1:25 - 1:50
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Mouse, Rat, Pig, Chicken
• Gene Accession No.: P12036
• Gene ID (Entrez): 4744
• Immunogen: Recombinant human neurofilament protein
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.

For Research Use Only